Overview

The Department of Microbiology and Immunology is represented by a diverse, yet highly interactive, faculty devoted to excellence in research as well as graduate and medical education. Faculty research is supported by outstanding postdoctoral associates, graduate students and research assistants. Research interests of the faculty are described in the links below.

MICROBIOLOGY

Francis Alonzo, PhD, Assistant Professor. Investigating the molecular mechanisms underlying Staphylococcus aureus disease processes and bacterial evasion of the host immune response.

Andrew K. Dingwall, PhD
, Associate Professor. Understanding the molecular, genetic and epigenetic mechanisms involving chromatin remodeling and nuclear receptor signaling that govern normal animal development and cancer progression.

Adam Driks, PhD, Professor. Molecular Microbiologist. Spore formation in Bacillus anthracis and Bacillus subtilis: roles in survival and pathogenesis, and defense against biological weapons.

David W. Hecht, MD,  Professor. Molecular Microbiologist. Mechanisms by which Bacteroides fragilis causes serious infections and acquires resistance to antimicrobial agents.

J. Paul O'Keefe, MD, Professor. Clinical Microbiologist. Clinical infectious diseases.

Karen L. Visick, PhD,  Professor. Microbial Geneticist. Vibrio fischeri-squid mutualism: a model for investigating symbiotic colonization by bacteria.

Richard M. Schultz, PhD, Professor Emeritus. Molecular Biologist. Enzymatic modification of histones and the regulation of gene expression.

Alan J. Wolfe, PhD, Professor. Molecular Geneticist. Cellular physiology and gene expression.

IMMUNOLOGY

Maskoor Choudry, PhD,  Professor. Department of Surgery. Intestine immunity and epithelial barrier function after alcohol exposure and injury.

John Clancy, Jr. PhD,  Professor Emeritus. Immunologist. Stem Cells and regenerative medicine.

Manuel O. Díaz, MD, Professor. Molecular Geneticist. Genetic abnormalities associated with neoplasia.

Kimberly E. Foreman, PhD,  Associate Professor. Tumor Immunologist. Notch Signaling in Breast Cancer.

Makio Iwashima PhD,  Associate Professor.  Molecular & Cellular Immunologist. Cell biology of T cell subsets.

Clodia Osipo, PhD, Associate Professor. Tumor immunology and investigating the significance of Notch signaling in breast cancer.

Katherine L. Knight, PhD, Professor & Chair. Molecular Immunologist. B-lymphocyte development and generation of the antibody repertoire.

Dennis Lanning, PhD, Research Assistant Professor. Molecular Immunologist Bacterial stimulation of GALT development.

Phong T. Le, PhD, Professor. Immunologist. T cell development and aging.

Caroline Le Poole, PhD, Associate Professor. Molecular/cellular Immunologist.  Autoimmunity and anti-tumor immunity to melanocytic cells.

Herbert L. Mathews, PhD, Professor. Immunologist. Immune dysregulation and homeostasis.

Liang Qiao, MD, Professor. Immunologist. Mucosal Immunity and Vaccine Development.

Pamela L. Witte, PhD,  Professor. Cellular Immunologist. The cellular and molecular regulation of B-lymphocyte development.

VIROLOGY

Susan C. Baker, PhD,  Professor. Molecular Virologist. The molecular biology and pathogenesis of coronaviruses.

Edward M. Campbell, PhD, Associate Professor. Intracellular immunity mediated by TRIM family proteins.

Thomas M. Gallagher, PhD, Professor. Virologist. Molecular Mechanisms of Virus Assembly and Entry.

Susan L. Uprichard, PhD, Associate Professor. Virologist. Elucidating the dynamics and molecular mechanisms that determine hepatitis virus infection outcome and therapeutic response.

Laboratories and Instrumentation

Each laboratory within the Department of Microbiology and Immunology is well equipped with current technology for research in microbiology, virology, and immunology.  Additionally common equipment is available  for use by all investigators including molecular imaging systems for documentation and quantitation of gels, real-time PCR thermocyclers, chromatography systems for biomolecule purification, spectrophotometers for quantitation and biophysical analysis of biomolecules, epifluorescence microscopes, high speed and ultra-centrifuges, scintillation counters, a dark room, and a environmental rooms.

Additionally, numerous core facilities are available on campus for use by investigators. Two of these Core facilities, the DNA Core and Transgenic Core Facility, are located within the Department of Microbiology and Immunology. Additional details of these Facilities are provided in the links below.

DNA Core Facility

The DNA Core Facility provides friendly service and timely DNA sequencing in an efficient manner by experienced molecular biologists. Sequencing is performed on an ABI® Prism 3100 4-capillary automated genetic analyzer with a capacity of about 35 samples in 24 hours. Sequencing is performed from single or double stranded DNA from plasmids and PCR products. Standard primers can be provided if necessary.

Transgenic Core Facility

The transgenic core facility provides services for a fee related to the production of transgenic mice using standard microinjection protocols. Additionally, custom animal surgeries can be performed and surgical training to research personnel can be provided as well. These services are available to the Loyola community and are provided in accordance with the IACUC policies and regulations.

Core Imaging Facility

The Core Imaging Facility is committed to excellence in research and education by providing expert technical assistance and instrumentation to support investigators requiring confocal and electron microscopy. This facility is equipped with both transmission (Hatachi® H-600 TEM) and scanning electron (JEOL® 840 A SEM) microscopes, four automatic ultramicrotomes, knife breakers, and all of the general equipment and facilities needed for tissue processing and darkroom work. The facility also maintains a Zeiss® LSM-510 laser scanning microscope interfaced to a Zeiss® Axiovert inverted microscope equipped with a variety of lens for high resolution work. Multiple channels allow simultaneous collection of images using the AR® 458/488 and HENE®543 lasers as well as bright-field DIC. Software for image acquisition and analysis is available with the instrument. In addition, there are two workstations with video input from either the SEM or fluorescent light microscope (Leitz® Orthoplan) for computerized image processing and analysis.

Flow Cytometry Facility

The Fluorescence-Activated Cell Sorter Facility (FACS Facility) at Loyola is a user-supported research core laboratory that provides flow cytometric and sorting services. The FACS is equipped with FACStar® Plus cell sorter, a FACS 420 cell sorter and a FACS Analyzer flow cytometer. The FACStar® Plus is equipped with an automatic cell deposition device that allows automated single cell cloning of any cell population based on user-defined parameters.

Molecular Core Facility

The Molecular Core Facility houses state-of-the-art instrumentation related to protein peptide microsequencing, solid phase peptide synthesis, amino acid analysis and high performance liquid chromatography and oligonucleotide synthesis and purification.