Laboratories

Section Chief

Stephen Kahn, PhD
Chemistry and Toxicology

Director

Earle W. Holmes, PhD
Special Chemistry and Immunoserology

Manager

Cindy Blakemore
Special Chemistry and Immunoserology

For additional information, please call customer service at 1-888-LML-LABS

Cardiovascular Risk

  • Apoprotein A1
  • Apoprotein B
  • Cholesterol
  • HDL Cholesterol
  • Homocysteine
  • LDL Cholesterol - Direct Measurement
  • Lipoprotein (a)
  • Triglycerides

Monoclonal Gammopathy

  • Immunofixation
  • Protein electrophoresis (serum and urine)
  • Quantitative immunoglobulins

Myocardial Infarction, Angina, and Arrhythmia

  • Amiodarone
  • CKMB
  • Digoxin
  • Myoglobin
  • Troponin I

Nutritional Assessment

  • Albumin
  • Prealbumin
  • Transferrin
  • Zinc (atomic absorption spectrophotometry)

Transplantation

  • Cyclosporin A
  • Prograf (FK506 or Tacrolimus)
  • OKT3 Antibody

The Clinical Hemostasis Laboratory offers a comprehensive test menu that includes specialized workups for abnormal PT and APTT patient results, seven-days-a-week laboratory operation, and individual consultative services with nationally known and internationally recognized coagulation specialists.

Director

Ameet Kini, MD, PhD

Manager

Kathleen McKenna

For additional information, please call customer service at 1-888-LML-LABS

Consultative Service

  • Individualized consultation with our coagulation specialists
  • Follow this link to reach the Coagulation synoptic templates

Panels

  • Prolonged APTT Workup
  • Prolonged PT Workup
  • Lupus Anticoagulant Panel
  • DIC Panel

Screening Tests

  • Prothrombin Time (PT)
  • Activated Partial Thromboplastin Time (APTT)
  • Fibrin Split Products (FSP)
  • Template Bleeding Time***
  • Thrombin Time (TT)

Coagulation Factors

  • Fibrinogen (clot based)
  • Fibrinogen Antigen
  • Factor II
  • Factor VII Activity
  • Factor VIII Activity
  • Factor IX Activity
  • Factor X Activity
  • Factor XI Activity
  • Factor XII Activity
  • HMW Kininogen (Fitzgerald Factor)
  • Prekallikrein (Fletcher Factor)
  • Factor XIII Screen (urea solubility)

Inhibitors

  • PT and/or APTT Mixing Study
  • Incubated Mixing Study (PT and/or APTT)
  • Factor VIII Inhibitor (Bethesda)
  • Lupus Anticoagulant
  • Prolonged APTT or PT Workup

Platelet Function

  • Platelet Aggregation*** (ADP - high and low dose, collagen, epinephrine, arachidonic acid, ristocetin - high and low dose)
  • Template Bleeding Time***

Fibrinolysis/DIC

  • Alpha-2 Plasmin Inhibitor Level
  • Antithrombin III Activity
  • D-dimer (quantitative or semi-quantitative)
  • Euglobulin Clot Lysis Time***
  • Fibrinogen (clot based)
  • FSP
  • Plasminogen
  • Protein C Activity

von Willebrand Disease

  • Factor VIII Activity
  • von Willebrand Factor (vWF) Activity (ristocetin cofactor)
  • von Willebrand Factor (vWF) Antigen (Factor VIII-related antigen)
  • von Willebrand Factor (vWF) Multimers

Thrombosis/Hypercoagulability

  • Anticardiolipin Antibodies (IgG, IgM, IgA)
  • Antithrombin III Activity or Antigen
  • Dilute Russell Viper Venom Time (DRVVT)
  • Euglobulin Clot Lysis Time***
  • Factor V Leiden (gene mutation)
  • Heparin-Induced Platelet Aggregation (HIPA)
  • Heparin-Induced Antibody Titer
  • Plasminogen Activity
  • Platelet Neutralization Procedure (PNP)
  • Protein C Activity or Antigen
  • Protein S Activity or Antigen (free and/or total)
  • Prothrombin Gene Mutation
  • Serotonin Release Assay (SRA)

Miscellaneous - Including ITP

  • Ecarin Clotting Time (Thrombin Inhibitor Assay)
  • Heparin Assay (anti-Xa assay) - specify LMW or unfractionated heparin
  • Reptilase Time
  • Platelet-Specific Antibody Screen (PLA1)
  • Platelet-Associated IgG/IgM (ITP)
  • Neonatal Alloimmune Thrombocytopenia (NAT)

*** Patient must come to the Medical Center for blood draw - appointment required.

Director

Guliz A. Barkan, MD, FIAC, FCAP

Manager

Grazina Chatt, CT (ASCP)

Services

  • Body fluids
  • Cervicovaginal smears
  • Exfoliative cytology
  • Fine needle aspirations (pathologist-performed)
  • Fine needle aspirations (staining and interpretation)

Special Procedures

  • Flow cytometry
  • Immunofluorescence
  • Immunohistochemistry
  • Molecular pathology
  • Special stains

Director

Kamran M. Mirza, MD PhD FCAP FACP

Associate Directors

Paul Schreckenberger, PhD
Ameet Kini, MD PhD

Interim Lab Manager (Microbiology/Molecular and HLA)

Roman G. Golash, M.S., M.B.A.

For additional information, please call customer service at 1-888-LML-LABS

Molecular Genetics

  • Factor V Leiden (PCR)
  • Prothrombin Gene Mutation (PCR)

Infectious Disease

  • HBV Qualitative (Tissue) (PCR)
  • HBV Quantitative (PCR)
  • HCV Qualitative (Tissue) (PCR)
  • HCV Monitor Quantitative (PCR)
  • HCV Genotyping
  • HIV Ultrasensitive (PCR)
  • HIV Monitor Quantitative (PCR)
  • CMV (Hybrid Capture)
  • Epstein-Barr Virus (for detection of viral genome in lymphoid lesions)

Engraftment Analysis

  • Chimerism

Director

Earle W. Holmes, PhD

Manager

Kathleen McKenna

For additional information, please call customer service at 1-888-LML-LABS

We provide a variety of tests for the evaluation of:

  • Hypothalamo-Pituitary function
  • Thyroid function
  • Adrenal function
  • Gonadal and Ovarian function
  • Diabetes

Adrenal Function Testing

  • Adrenal steroids
  • Catecholamines and metabolites
  • Steroid precursors

Gonadal Function Testing

  • Free & weakly bound testosterone
  • Sex hormone binding globulin

Peptide Hormone Quantitation

  • Adrenal corticotropic hormone
  • C-peptide
  • Gastrin
  • Growth Hormone
  • Insulin
  • Parathyroid hormone
  • Prolactin

Reproductive Hormone Testing

  • 3:00 p.m. results for specimens received by 10:00 a.m.
  • Estradiol
  • FSH
  • HCG
  • LH
  • Progesterone

Thyroid Function Testing

  • Anti-TPO Antibodies
  • Free T4
  • Free T3
  • Thyroglobulin
  • Thyroxine binding globulin
  • TSH, T4, T3

Director

Ameet Kini, MD, PhD

Manager

Kathleen McKenna

For additional information, please call customer service at 1-888-LML-LABS

We perform a number of tests including:

  • CD34 Hematopoietic Progenitor Cell Counts
  • CD4/CD8 Monitoring
  • Consultative Service
  • Leukemia/Lymphoma Immunophenotyping
  • Leukoreduced Blood Product Monitoring
  • PNH Detection
  • T and B Cell Subset Analysis

Director

Earle W. Holmes, PhD

Manager

Kathleen McKenna

For additional information, please call customer service at 1-888-LML-LABS

We provide a variety of tests for the diagnosis and management of:

  • HIV, Hepatitis, and other infectious diseases
  • Autoimmune Diseases
  • Inhalant and Food Allergies

Allergy

  • Custom allergen-specific IgE panels
  • Food screens
  • Inhalant screens
  • Latex

Antiphospholipid Disease

  • Anticardiolipin antibodies
  • Lupus-type inhibitor studies

Autoimmune Disease

  • Liver
  • Thyroid

Infectious Disease Serology

Rheumatology

  • Anti-DNA antibodies
  • Antinuclear antigen (ANA)
  • Antineutrophil cytoplasmic antibodies (ANCA)
  • Extractable nuclear antigen (ENA)

 

 

 

Director

Paul Schreckenberger, PhD

Manager

TBA

For additional information, please call customer service at 1-888-LML-LABS

Antimicrobial Susceptibility Testing

Penicillin-resistant pneumococci

  • MIC/MBC
  • Vancomycin-resistant enterococci
  • Vancomycin intermediate staphylococcus aureus

Epidemiology Testing

  • Pulse Gel Field Electrophoresis available for isolate characterization

Microbiology

  • Bordetella: direct detection, culture, and identification
  • Culture and identification of E. coli O157:H7
  • Legionella: direct detection, culture, and identification
  • Identification of gram-positive cocci and rods

Mycobacteriology

  • Rapid DNA probe identification
  • Susceptibility testing of M. tuberculosis

Mycology

  • Identification of dermatophytes of colonies or broth culture
  • Identification of unusual fungal isolates
  • Fungal susceptibility testing

Parasitology

  • Giardia screen
  • Cryptosporidium screen
  • Malaria identification
  • Ova and parasite exam
  • Pneumocystis carinii screen

Serodiagnosis of Viral and Bacterial Infections

  • HIV
  • Hepatitis A, B, and C
  • IgG & IgM antibody to CMV, herpes, toxoplasma, and EBV
  • Immune status for rubella, measles, and varicella
  • Lyme disease

Virology

  • Clostridium difficile toxin assay
  • Direct detection of RSV, influenza A, herpes simplex, varicella zoster, rotavirus
  • Herpes simplex virus typing
  • Rapid shell vials for CMV and respiratory viruses

Director

Ameet Kini, MD, PhD

Manager

Sandra Chakonas

For additional information, please call customer service at 1-888-LML-LABS

Consultative Services

  • Bone marrow
  • Lymph nodes
  • Special cytochemical stains

Investigation of Hemoglobinopathies

  • Hemoglobin electrophoresis (Alkaline)
  • High performance liquid chromatography (HPLC)

Investigation of Hemolytic Anemias

  • Acidified serum lysis tests (HAMS)
  • Heint body screen
  • Osmotic fragility
  • Sucrose hemolysis

Anatomic Pathology is composed of a team of academic, board certified pathologists. We are an all-inclusive laboratory that also offers diagnostic expertise and immediate interpretations in a wide variety of specialty and subspecialty areas. Electronic methods of communication provide efficient and rapid reporting of all diagnoses.

Director

Stefan Pambuccian MD

Manager

Steven G. Pinheiro, MBA, MLS(ASCP)DLM

Expert and immediate consultations are available with phone or fax response For additional information, please call customer service at 1-888-LML-LABS.

Autopsy

  • General
  • All neurological diseases, including:
    • Alzheimer's disease
    • Neurodegenerative disorders
  • Pediatric

Cytopathology

Special Procedures Available

  • DNA ploidy
  • Flow cytometry
  • Immunofluorescence
  • Immunohistochemistry
  • Molecular pathology
  • Special stains

Surgical Pathology

  • Breast Pathology - Roberto Gamez, MD
  • Cardiac Pathology - Maria M. Picken, MD, PhD
  • Dermatopathology - Kelli Ann Hutchens, MD
  • Gastrointestinal Pathology - Mohammed Atieh, DO
  • Genitourinary Pathology - Maria M. Picken, MD, PhD
  • Gynecologic Pathology - Xiuzhen Duan, MD, PhD
  • Head and Neck Pathology - Swati Mehrotra, MD
  • Hematopathology - Ameet Kini, MD, PhD and Milind Velankar, MD
  • Infectious Disease Pathology
  • Liver Pathology
  • Neuropathology (including nerve and muscle biopsy) - Ewa Borys, MD
  • Ophthalmic Pathology
  • Oral Pathology - Swati Mehrotra, MD
  • Orthopedic Pathology - Dariusz Borys, MD
  • Pediatric Pathology (including perinatal and placental) - Dariusz Borys, MD
  • Podiatric Pathology - Dariusz Borys, MD
  • Pulmonary Pathology - Swati Mehrotra, MD
  • Renal Pathology - Maria M. Picken, MD, PhD, Ewa Borys, MD
  • Transplant (heart, liver, lung, kidney) - Maria M. Picken, MD, PhD
  • Special Stains - Xiuzhen Duan, MD, PhD

Director

Phillip J. DeChristopher, MD, PhD

Manager

Linda Leung

Coordinator

Anne Link

For additional information, please call customer service at 1-888-LML-LABS

Director

Stephen Kahn, PhD

Manager

Cindy Blakemore

For additional information, please call customer service at 1-888-LML-LABS

Drugs of Abuse

Clinical, emergency, and workplace drug testing.

  • Amphetamines
  • Barbiturates
  • Benzodiazepines
  • Cannabinoids
  • Cocaine metabolite
  • Opiates
  • Phencyclidine

Screening by enzyme immunoassay. Confirmation by gas chromatography/mass spectroscopy.

Gases

  • Carboxyhemoglobin (cooximetry profile)

Lead Analysis

  • Whole blood lead (atomic absorption spectrophotometry)

Therapeutic Drugs

  • Acetaminophen
  • Salicylate
  • Lithium
  • Methotrexate
  • Antiarrythmics including amiodarone, digoxin, lidocaine, procainamide, and NAPA
  • Antibiotics including amikacin, gentamicin, tobramycin, and vancomycin
  • Antiepileptics including carbamazepine, ethosuximide, phenobarbital, phenytoin, primidone, and valproic acid
  • Immunosuppressants including cyclosporine A and Prograf (FK506 or Tacrolimus)
  • Theophylline

Volatiles

  • Ethanol
  • Volatile screen including ethanol, methanol, isopropanol, and other compounds

The human leukocyte antigen (HLA) complex, located on Chromosome 6q21, comprises genes encode two distinct classes of highly polymorphic cell surface antigen. Class I HLA-A, -B, -C antigens are expressed ubiquitously on nucleated cells, whereas the class II HLA-DR, -DQ, -DP antigens are normally expressed on B cells, monocytes, macrophage, dendritic cells and on activated T cells. An extensive sequence polymorphism is present in the second exon of Class II loci and in the second and third exons of Class I loci. These polymorphic regions encode the peptide-binding groove of the HLA molecules and are important to transplantation, disease susceptibility and drug hypersensitivity.

The Clinical Histocompatibility (HLA) Laboratory at Loyola University Medical Center is accredited by College of American Pathologists (CAP) and American Society of Histocompatibility and Immunogenetics (ASHI). The laboratory provides the state-of-art immunogenetics testing services to support comprehensive transplant programs and other clinical services at Loyola University Medical Center, including:

  • Solid organ (Kidney, Liver, Heart, Lung and Pancreas and combined organs) transplantation
  • Allogeneic Hematopoietic stem cell/bone marrow  transplantation
  • Other clinical purposes: disease associations, drug hypersensitivity and platelet transfusions, etc.

In addition, the laboratory provides the massive educational opportunities including observations, hands-on experiences, lectures and case studies for medical students, residents and fellows in Stritch School of Medicine, Loyola University Chicago. The trainees acquire knowledge on Immunogenetics and transplant immunology in clinical applications during their rotations and selective courses.   

Test offered:

HLA Typing

Diversity of HLA molecules represents a major barrier to organ and tissue transplantation between individuals and also has been implicated in susceptibility or resistance to a variety of autoimmune, infectious, and oncologic disorders. HLA typing identifies the unique constellation of HLA antigens for an individual. HLA-class I (A, B, C) and class II (DR, DQ, DP) typing is performed by DNA-based molecular diagnostic techniques.  At the current stage, the laboratory provides low/intermediate HLA typing using PCR-sequence-specific oligonucleotide probe hybridization (SSOP) in house. High resolution HLA typing is sent out now.   

HLA antibody Screening (PRA)

Preformed anti-HLA antibodies are associated with acute rejection of mismatched solid organ and stem cell grafts, failure of platelet transfusions. Patients may become sensitized to HLA antigens through pregnancies, blood transfusions or previous transplantations and develop HLA antibodies. The laboratory performs this test on Luminex phenotype antigen platform that uses a panel of HLA class I and class II antigens isolated from human cell lines coupled to microbeads. These HLA antigens are common and frequent HLA antigens in human populations.  Any HLA antibodies present in the test serum bind to the antigens on the microbeads will be detected by a Luminex analyzer. Results are reported as percentage of panel reactive antibodies (PRA) which indicate the breadth of HLA antibody reactivity in populations and give an estimate of the likelihood of finding a suitable donor.

HLA antibody identification

HLA class I and class II antibody specificity identification is critical in transplant patients. They are tested by Luminex single antigen platform that uses a panel of recombinant allele specific HLA class I and class II antigens coated on microbeads.  Any HLA antibodies present in the test serum bind to the specific HLA single antigens on the microbeads will be detected by a Luminex analyzer.  Thus, HLA class I and class II antibody specificities can be detected accurately. The Class I and Class II antibody screening and identification tests help to identify the most compatible donors for a patient need organ, mismatch stem cell transplantation or platelet transfusion.

 Donor Specific HLA antibodies (DSA)

Pre-formed or de novo donor specific antibodies (DSA) is the major risk factor of acute and chronic graft rejection. Using Luminex single antigen platform to accurately detect HLA antibody identification, DSA can be defined for specific donors with the available donor HLA typing. This testing will help clinician to monitor the patient’s under immunomodulation and post-transplant immune status for therapeutic purposes.

T- and B-cell Flow cytometry crossmatch

Crossmatches detect donor specific antibodies by incubating a donor’s lymphocytes with a recipient’s serum. Positive crossmatches detected by flow cytometry suggest the presence of preformed anti-donor antibodies and indicate a risk of acute rejection and damage to the graft. Crossmatch results are interpreted in combination of the patient's disease status, sensitization history, HLA antibody testing and the quality of the donor cells.  

Director, Technical Supervisor and Clinical Consultant

Zeying Du, MD, PhD, D(ABHI)
Phone: 708-216-9028

General Supervisor
Jaishree Patel CHS(ABHI)
Phone: 708-216-3626

Contact Information

Hours of Operation:
Monday through Friday, 8:00 am to 4:30 pm
Phone: 708-216-3626
Fax: 708-216-1047


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Director

Phillip J. DeChristopher, MD, PhD

Manager

Linda Leung

For additional information, please call customer service at 1-888-LML-LABS

  • Prenatal Testing
  • Pre-Transfusion Testing
  • Stem Cell Collection
  • Therapeutic Apheresis
  • Therapeutic Phlebotomy
  • Autologous Red Blood Cell Collection