Sample Submission Requirements

  • Please submit samples in sterile 1.5 ml microcentrifuge tubes.
  • Please clearly label each sample on tube using the initials of the investigator and consecutive numbers (e.g. JR-01).
  • Numbers should never be repeated even in separate submissions, so the sample label will serve to track the total number of samples an investigator has submitted.
  • If submitting samples on a plate, the plate should be sealed properly and labeled using the following convention: P.I. initials_Date_Sample 1 to Sample n.
  • When delivering samples please pack on dry ice to ensure the samples remain frozen to prevent any potential degradation.
  • Complete the sample submission form found in the navigation menu.
  • Every sample submission must be accompanied by the Sample Information Form found in the navigation menu. Please download and email to gkuffel@luc.edu.
  • Please check the table below to get information corresponding to the specific application requested.

Application

Input AmountNotes
16S rRNA Gene Amplicon min of 20 ul in nuclease free water or TE buffer Please contact facility for correct adapter sequences if performing amplicon PCR before submission.
Custom Amplicon min of 20 ul in nuclease free water or TE buffer Please contact facility for correct adapter sequences if performing amplicon PCR before submission.
Microbial Whole Genome min of 10 ul in nuclease free water, at least 5 ng required Protocol is optimized for a 260/280 > 1.8.
Microbial RNASeq 100 ng-1 ug total RNA in RNAse-free water or TE Buffer Samples must be treated with DNAse. Sample should be free of salts (e.g. Mg) or organics (e.g. ethanol).
Micro RNASeq min of 2 ug of total RNA in 25 ul nuclease free water Include a DNase step with the RNA Isolation method. RNA isolation method must retain nucleotides < 30 nt (e.g. miRNeasy).
ImmunoSeq Assay dependent, contact for details Protocol is optimized for a 260/280 > 1.8.