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John Callaci
Associate Professor
Primary Department
Orthopaedic Surgery
Joint Department
Cell and Molecular Physiology
Advanced Degrees
Chicago Medical School/Finch University of Health Sciences , North Chicago , IL , United States
Interests: Effects of Binge Alcohol Exposure on Skeletal Biology

My research is focused on the biochemical, biomechanical and molecular effects of alcohol on the skeleton. Previous studies performed in my laboratory using a binge model of alcohol consumption, identified that bone resorptive activity increases after acute binge alcohol exposure, which is at least partially responsible for bone loss observed after binge alcohol treatment. Alcohol-induced bone loss can be effectively blocked with anti-resorptive drugs, suggesting a possible therapeutic response to alcoholic osteopenia. We have also shown that binge alcohol exposure has an additive effect on bone loss caused by other insults to the skeleton such as ovariectomy-induced estrogen depletion. Research efforts identifying biomarkers of early alcohol-mediated bone damage in adolescent and adult ovariecotomized rodents using gene expression array technology allowed us to identify novel mechanisms underlying bone damage caused by alcohol and early markers of bone loss that can be used to identify patients at risk for osteoporosis years prior to conventional diagnostics. Our current research focuses on the mechanisms responsible for fracture non-union related to alcohol abuse. We have identified that alcohol deregulates key signaling pathways that control the fracture healing process. We are using this information to develop therapeutic approaches to treat patients with non healing fractures relayed to alcohol abuse and other causes such as diabetes.

View a partial list of Dr. Callaci's publications through the National Library of Medicine's PubMed online database.
Wound Healing
Natoli, RM; Yu, H; Meislin, MC; Abbasnia, P; Roper, P; Vuchkovska, A; Xiao, X; Stock, SR; Callaci, JJAlcohol exposure decreases osteopontin expression during fracture healing and osteopontin-mediated mesenchymal stem cell migration in vitro. Journal of orthopaedic surgery and research 2018 ;13(1)
Bratton, A; Eisenberg, J; Vuchkovska, A; Roper, P; Callaci, JJEffects of Episodic Alcohol Exposure on BMP2 Signaling During Tibia Fracture Healing. Journal of orthopaedic trauma 2018 ;32(6)
Roper,P. M.; Abbasnia,P.; Vuchkovska,A.; Natoli,R.; Callaci,J. J.Alcohol-related deficient fracture healing is associated with activation of FoxO transcription factors in mice Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2016 ; :
Hoscheit,M.; Conner,G.; Roemer,J.; Vuckovska,A.; Abbasnia,P.; Vana,P.; Shankar,R.; Callaci,J.Burn Injury Has Skeletal Site-Specific Effects on Bone Integrity and Markers of Bone Remodeling Journal of burn care & research : official publication of the American Burn Association 2016 ; :
Driver,J.; Weber,C. E.; Callaci,J. J.; Kothari,A. N.; Zapf,M. A.; Roper,P. M.; Borys,D.; Franzen,C. A.; Gupta,G. N.; Wai,P. Y.; Zhang,J.; Denning,M. F.; Kuo,P. C.; Mi,Z.Alcohol inhibits osteopontin-dependent transforming growth factor-beta1 expression in human mesenchymal stem cells The Journal of biological chemistry 2015 ;290(16):9959-9973
Lauing,K. L.; Sundaramurthy,S.; Nauer,R. K.; Callaci,J. J.Exogenous activation of Wnt/beta-catenin signaling attenuates binge alcohol-induced deficient bone fracture healing Alcohol and Alcoholism (Oxford, Oxfordshire) 2014 ;49(4):399-408
Cappello,T.; Nuelle,J. A.; Katsantonis,N.; Nauer,R. K.; Lauing,K. L.; Jagodzinski,J. E.; Callaci,J. J.Ketorolac administration does not delay early fracture healing in a juvenile rat model: a pilot study Journal of pediatric orthopedics 2013 ;33(4):415-421
Beck,J.; Evans,D.; Tonino,P. M.; Yong,S.; Callaci,J. J.The biomechanical and histologic effects of platelet-rich plasma on rat rotator cuff repairs. American Journal of Sports Medicine 2012 ;40(9):2037-2044
Lauing,K. L.; Roper,P. M.; Nauer,R. K.; Callaci,J. J.Acute alcohol exposure impairs fracture healing and deregulates beta-catenin signaling in the fracture callus Alcoholism, Clinical and Experimental Research 2012 ;36(12):2095-2103
Obermeyer,T. S.; Yonick,D.; Lauing,K.; Stock,S. R.; Nauer,R.; Strotman,P.; Shankar,R.; Gamelli,R.; Stover,M.; Callaci,J. J.Mesenchymal stem cells facilitate fracture repair in an alcohol-induced impaired healing model Journal of orthopaedic trauma 2012 ;26(12):712-718
Jung,M. K.; Callaci,J. J.; Lauing,K. L.; Otis,J. S.; Radek,K. A.; Jones,M. K.; Kovacs,E. J.Alcohol exposure and mechanisms of tissue injury and repair Alcoholism, Clinical and Experimental Research 2011 ;35(3):392-399
Sears,B. W.; Volkmer,D.; Yong,S.; Himes,R. D.; Lauing,K.; Morgan,M.; Stover,M. D.; Callaci,J. J.Binge alcohol exposure modulates rodent expression of biomarkers of the immunoinflammatory response to orthopaedic trauma. Journal of Bone & Joint Surgery - American Volume 2011 ;93(8):739-749
Volkmer,D. L.; Sears,B.; Lauing,K. L.; Nauer,R. K.; Roper,P. M.; Yong,S.; Stover,M.; Callaci,J. J.Antioxidant therapy attenuates deficient bone fracture repair associated with binge alcohol exposure Journal of orthopaedic trauma 2011 ;25(8):516-521
Callaci,J. J.; Himes,R.; Lauing,K.; Roper,P.Long-term modulations in the vertebral transcriptome of adolescent-stage rats exposed to binge alcohol. Alcohol & Alcoholism 2010 ;45(4):332-346
Sears,B. W.; Volkmer,D.; Yong,S.; Himes,R. D.; Lauing,K.; Morgan,M.; Stover,M. D.; Callaci,J. J.Correlation of measurable serum markers of inflammation with lung levels following bilateral femur fracture in a rat model Journal of inflammation research 2010 ;2010(3):105-114
Mikolyzk,D. K.; Wei,A. S.; Tonino,P.; Marra,G.; Williams,D. A.; Himes,R. D.; Wezeman,F. H.; Callaci,J. J.Effect of corticosteroids on the biomechanical strength of rat rotator cuff tendon. Journal of Bone & Joint Surgery - American Volume 2009 ;91(5):1172-1180
Sears,B. W.; Stover,M. D.; Callaci,J.Pathoanatomy and clinical correlates of the immunoinflammatory response following orthopaedic trauma Journal of the American Academy of Orthopaedic Surgeons 2009 ;17(4):255-265
Emanuele,N. V.; Emanuele,M. A.; Morgan,M. O.; Sulo,D.; Yong,S.; Kovacs,E. J.; Himes,R. D.; Callaci,J. J.Ethanol potentiates the acute fatty infiltration of liver caused by burn injury: prevention by insulin treatment. Journal of Burn Care & Research 2009 ;30(3):482-488
Callaci,J. J.; Himes,R.; Lauing,K.; Wezeman,F. H.; Brownson,K.Binge alcohol-induced bone damage is accompanied by differential expression of bone remodeling-related genes in rat vertebral bone. Calcified tissue international 2009 ;84(6):474-484
Lauing,K.; Himes,R.; Rachwalski,M.; Strotman,P.; Callaci,J. J.Binge alcohol treatment of adolescent rats followed by alcohol abstinence is associated with site-specific differences in bone loss and incomplete recovery of bone mass and strength. Alcohol 2008 ;42(8):649-656
Himes,R.; Wezeman,F. H.; Callaci,J. J.Identification of novel bone-specific molecular targets of binge alcohol and ibandronate by transcriptome analysis. Alcoholism: Clinical & Experimental Research 2008 ;32(7):1167-1180
Pinzur,M. S.; Beck,J.; Himes,R.; Callaci,J.Distal tibiofibular bone-bridging in transtibial amputation Journal of Bone & Joint Surgery, American Volume 2008 ;90(12):2682-2687
Wezeman,F. H.; Juknelis,D.; Himes,R.; Callaci,J. J.Vitamin D and ibandronate prevent cancellous bone loss associated with binge alcohol treatment in male rats. Bone 2007 ;41(4):639-645
Callaci,J. J.; Juknelis,D.; Patwardhan,A.; Wezeman,F. H.Binge alcohol treatment increases vertebral bone loss following ovariectomy: compensation by intermittent parathyroid hormone. Alcoholism: Clinical & Experimental Research 2006 ;30(4):665-672
Wei,A. S.; Callaci,J. J.; Juknelis,D.; Marra,G.; Tonino,P.; Freedman,K. B.; Wezeman,F. H.The effect of corticosteroid on collagen expression in injured rotator cuff tendon. Journal of Bone & Joint Surgery - American Volume 2006 ;88(6):1331-1338
Callaci,J. J.; Juknelis,D.; Patwardhan,A.; Sartori,M.; Frost,N.; Wezeman,F. H.The effects of binge alcohol exposure on bone resorption and biomechanical and structural properties are offset by concurrent bisphosphonate treatment. Alcoholism: Clinical & Experimental Research 2004 ;28(1):182-191
Wezeman,F. H.; Juknelis,D.; Frost,N.; Callaci,J. J.Spine bone mineral density and vertebral body height are altered by alcohol consumption in growing male and female rats. Alcohol 2003 ;31(1-2):87-92